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Table 3 Characteristics of the purified recombinant xylanases

From: Cloning of novel bacterial xylanases from lignocellulose-enriched compost metagenomic libraries

Xylanase

XYL21

XYL32

XYL35

XYL38

XYL40

Domain organization

[GH10]-[FN3]-[FN3]-[CBM2]

[GH8]

[GH11]

[GH10]

[GH11]-[CBM60]

Size (aa)a

602

385

196

370

325

Mw (kDa)a

63.2

44.3

24

41.9

34.9

pIa

7.5

5.4

9.8

6.9

8.1

pH optimum

5.5

5.5

7.0

6.5

6.5

Temp. optimum

60

40

70

80

70

XOS produced from GX

X2/X3

X2/X3

X2–X4

X1/X2

X2

GX (U/mg)

17.2

1048

293

7.1

281

AX (U/mg)

23.8

1551

1062

13.8

399

DiFMUX2 (U/mg)

0.110

0.0072

10.0

0.106

1.94

Symbol used in legends

  1. aFigures presented for molecular weight and pI were calculated based on the amino acid sequences of the native secreted proteins excluding the purification tag
  2. GX birchwood glucuronoxylan, AX wheat straw arabinoxylan