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Table 3 Characteristics of the purified recombinant xylanases

From: Cloning of novel bacterial xylanases from lignocellulose-enriched compost metagenomic libraries

Xylanase XYL21 XYL32 XYL35 XYL38 XYL40
Domain organization [GH10]-[FN3]-[FN3]-[CBM2] [GH8] [GH11] [GH10] [GH11]-[CBM60]
Size (aa)a 602 385 196 370 325
Mw (kDa)a 63.2 44.3 24 41.9 34.9
pIa 7.5 5.4 9.8 6.9 8.1
pH optimum 5.5 5.5 7.0 6.5 6.5
Temp. optimum 60 40 70 80 70
XOS produced from GX X2/X3 X2/X3 X2–X4 X1/X2 X2
GX (U/mg) 17.2 1048 293 7.1 281
AX (U/mg) 23.8 1551 1062 13.8 399
DiFMUX2 (U/mg) 0.110 0.0072 10.0 0.106 1.94
Symbol used in legends
  1. aFigures presented for molecular weight and pI were calculated based on the amino acid sequences of the native secreted proteins excluding the purification tag
  2. GX birchwood glucuronoxylan, AX wheat straw arabinoxylan