Skip to main content

Table 1 Products in the culture supernatants used for determination of enzymatic activities for strains H110 and TB8

From: Production of β-1,3-glucanase and chitosanase from clostridial strains isolated from the soil subjected to biological disinfestation

Strain Substrate Products in the culture supernatant (mmol/l)
Acetate Butyrate Ethanol n-Butanol
H110 None (PY) 0.73 ± 0.09 3.60 ± 0.05 1.63 ± 0.05
Curdlan 4.94 ± 0.58 15.9 ± 2.11 0.78 ± 0.06 14.9 ± 0.13
Yeast glucana 0.42 ± 0.01 12.7 ± 0.45 1.45 ± 0.05 1.42 ± 0.07
Chitosan 4.89 ± 0.22 19.5 ± 0.21 0.72 ± 0.07
TB8 None (PY) 0.92 ± 0.05 2.90 ± 0.01 0.85 ± 0.04
Curdlan 4.28 ± 0.01 22.0 ± 0.49 5.68 ± 0.52
Yeast glucana 0.87 ± 0.04 14.0 ± 0.85 1.08 ± 0.07 0.62 ± 0.09
Chitosan 4.55 ± 0.20 24.7 ± 0.82 0.70 ± 0.24
  1. Concentrations of substrate, 0.5% (w/v). Both the strains produced H2 and CO2 from all substrates
  2. Cultivated for 7 days for all substrates and the supernatant of each culture was used for determination of enzymatic activities shown in Table 2
  3. All data are mean values (± SD) of duplicate experiments
  4. not detected
  5. aGlucan from black yeast (Tokyo Chemical Industry Co., Ltd)