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Table 1 Products in the culture supernatants used for determination of enzymatic activities for strains H110 and TB8

From: Production of β-1,3-glucanase and chitosanase from clostridial strains isolated from the soil subjected to biological disinfestation

Strain

Substrate

Products in the culture supernatant (mmol/l)

Acetate

Butyrate

Ethanol

n-Butanol

H110

None (PY)

0.73 ± 0.09

3.60 ± 0.05

1.63 ± 0.05

Curdlan

4.94 ± 0.58

15.9 ± 2.11

0.78 ± 0.06

14.9 ± 0.13

Yeast glucana

0.42 ± 0.01

12.7 ± 0.45

1.45 ± 0.05

1.42 ± 0.07

Chitosan

4.89 ± 0.22

19.5 ± 0.21

0.72 ± 0.07

TB8

None (PY)

0.92 ± 0.05

2.90 ± 0.01

0.85 ± 0.04

Curdlan

4.28 ± 0.01

22.0 ± 0.49

5.68 ± 0.52

Yeast glucana

0.87 ± 0.04

14.0 ± 0.85

1.08 ± 0.07

0.62 ± 0.09

Chitosan

4.55 ± 0.20

24.7 ± 0.82

0.70 ± 0.24

  1. Concentrations of substrate, 0.5% (w/v). Both the strains produced H2 and CO2 from all substrates
  2. Cultivated for 7 days for all substrates and the supernatant of each culture was used for determination of enzymatic activities shown in Table 2
  3. All data are mean values (± SD) of duplicate experiments
  4. not detected
  5. aGlucan from black yeast (Tokyo Chemical Industry Co., Ltd)