Fig. 5

Application of the Agrobacterium growth inhibition sequence in screening recombinant clones. A Structure of the destination vector pCB301-attL-SC15P, which shows the elements between LB and RB; B Growth of Agrobacterium at 2 days post-transformation with pCB301-attL-SC15P (a), pDonor-Zeocin-CI (b), pDonor-Zeocin-Avh241 (c), LR recombination reaction product between pCB301-attL-SC15P and pDonor-Zeocin-CI (d), LR recombination reaction product between pCB301-attL-SC15P and pDonor-Zeocin- Avh241 (e); scale bars = 4 mm. C Identification of the colonies by PCR: 1 to 20 of (c) and (e) represent the colonies picked from the (c) and (e) plate in the B panel; N represents the negative control that used ddH₂O as its template; the DNA marker is DL5000