Fig. 1From: Characterization of recombinant laccase from Trametes versicolor synthesized by Arxula adeninivorans and its application in the degradation of pharmaceuticalsIsolation of different laccase genes from T. versicolor and P. cinnabarinus for the expression in A. adeninivorans G1212 using the strong constitutive A. adeninivorans derived TEF1 promoter (PTEF1) and the PHO5 terminator from S. cerevisiae (TPHO5). Laccase gene expressing strains were preselected using ABTS and guaiacol agar plates. For the quantification of secreted laccase, the selected strains were grown in liquid minimal medium for 3 days. Laccase activity of the supernatant was estimated after centrifugation using ABTS as substrateBack to article page