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Table 2 Description of designed species-specific primers description

From: Development of quantitative PCR for the detection of Alkalilimnicola ehrlichii, Thioalkalivibrio sulfidiphilus and Thioalkalibacter halophilus in gas biodesulfurization processes

Primer ID Primer sequence E. coli position Theoretical Tm (°C) Length (bp) GC content (%)
Thio-6F AGG GCT AGA GTT TGG TAG 647 52 18 50
Thio-8R AGA GGC ATA ATC CTC CCA 834 54 18 50
Alkali-4AF GTT AAT AGC CGT GGG TCT 462 54 18 50
Alkali-6BR TAC CAG ACT CTA GCC CGA 646 56 18 56
Tab-137-G_F CTT AGG TGG GGG ATA ACA CG 137 57 20 55
Tab-210R ATC CTT TGG CGC GAG GTC CG 210 65 20 65
  1. F, forward; R, reverse; Thio, Thioalkalivibrio spp.; Alkali, Alkalilimnicola ehrlichii; Tab, Thioalkalibacter halophilus