Development of quantitative PCR for the detection of Alkalilimnicola ehrlichii, Thioalkalivibrio sulfidiphilus and Thioalkalibacter halophilus in gas biodesulfurization processes

AMB Express

Table 2 Description of designed species-specific primers description

Primer ID	Primer sequence	E. coli position	Theoretical T_m (°C)	Length (bp)	GC content (%)
Thio-6F	AGG GCT AGA GTT TGG TAG	647	52	18	50
Thio-8R	AGA GGC ATA ATC CTC CCA	834	54	18	50
Alkali-4AF	GTT AAT AGC CGT GGG TCT	462	54	18	50
Alkali-6BR	TAC CAG ACT CTA GCC CGA	646	56	18	56
Tab-137-G_F	CTT AGG TGG GGG ATA ACA CG	137	57	20	55
Tab-210R	ATC CTT TGG CGC GAG GTC CG	210	65	20	65

F, forward; R, reverse; Thio, Thioalkalivibrio spp.; Alkali, Alkalilimnicola ehrlichii; Tab, Thioalkalibacter halophilus