Table 1 Strains, plasmids and primers used in this study
Strains, plasmids, primers | Description | Reference/source |
|---|---|---|
Strains | ||
P. antarctica GB-4(0) | Wild type strain isolated from rice husks and stocked in our laboratory | Kitamoto et al. (2011) |
P. antarctica GB-4(0) ∆PaEMT1 | PaEMT1 deleted strain | In this study |
Plasmids | ||
pAG25 | Dominant marker gene deletion cassette: pAgTEF1-natMX-tAgTEF1, clonNAT and ampicillin resistance | Goldstein and McCusker (1999) |
pUXV1_neo | Expression plasmid, glyceraldehyde-3P-dehydrogenase (gap) promoter, geneticin (G418) and ampicillin resistance | Watanabe et al. (2016) |
pUXV1_neo-PaEMT1 | Inserted PaEMT1 from P. antarctica T-34 into pUVX1_neo | Morita et al. (2013b) |
Primers | ||
PaEMT1_up_F1 | GAAGAGGCAATGGTGTTGCT | In this study |
PaEMT1_up_R1 | CGGCGGGGACAAGGCAAGCTATTTGCTTGAGCGATATGCTTTTG | In this study |
NAT_F1 | AGCATATCGCTCAAGCAAATAGCTTGCCTTGTCCCCGCCGGGTCAC | In this study |
NAT_R1 | GTTTAATCAGGACAAGGCGTTCGACACTGGATGGCGGCGTTAGTATCG | In this study |
PaEMT1_down_F1 | ACGCCGCCATCCAGTGTCGAACGCCTTGTCCTGATTAAACTGCTG | In this study |
PaEMT1_down_R1 | CAATGTCATCCACAGCACTC | In this study |
PaEMT1_inner_F1 | CTGCATCGATTGATCCATTG | In this study |
PaEMT1_inner_R1 | GTCGAACCACTGCGACAGGA | In this study |
PaEMT1_up_F2 | ATTGCTGACCATCATGGTGC | In this study |
NATfragment_R1 | CACGTCAAGACTGTCAAGGA | In this study |
PaCLE1_qPCR_F | TTCCGGACCATGAACACCCG | In this study |
PaCLE1_qPCR_R | AGGAGGAAGCACGTGTTGGG | In this study |
Actin_qPCR_F | CAGTCGTCTGCGCTCGAGAA | In this study |
Actin_qPCR_R | CGGATGTCCAGGTCGCACTT | In this study |