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Fig. 6 | AMB Express

Fig. 6

From: Deficiency of biodegradable plastic-degrading enzyme production in a gene-deletion mutant of phyllosphere yeast, Pseudozyma antarctica defective in mannosylerythritol lipid biosynthesis

Fig. 6

SDS-PAGE and PaE western blot analysis of culture supernatant and cell-free extract of GB-4(0) and strain ∆PaEMT1. a Cell growth. b SDS-PAGE and PaE detection through western blot analysis. M, marker; P, purified PaE; Lane 1, supernatant of wild-type; Lane 2, soluble fraction of wild-type; Lane 3, insoluble fraction of wild-type; Lane 4, soluble fraction after 0.01% TritonX-100 treatment of wild-type; Lane 5, insoluble fraction after 0.01% TritonX-100 treatment of wild-type; Lane 6, supernatant of ∆PaEMT1; Lane 7, soluble fraction of ∆PaEMT1; Lane 8, insoluble fraction of ∆PaEMT1; Lane 9, soluble fraction after 0.01% TritonX-100 treatment of ∆PaEMT1; Lane 10, insoluble fraction after 0.01% TritonX-100 treatment of ∆PaEMT1. The amount of each sample loaded in the gel was 20 μL for CBB staining and western blotting. Error bars show standard deviations

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