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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: Deficiency of biodegradable plastic-degrading enzyme production in a gene-deletion mutant of phyllosphere yeast, Pseudozyma antarctica defective in mannosylerythritol lipid biosynthesis

Fig. 3

PaE production by strain ∆PaEMT1 harboring PaEMT1 in 3×FMM medium supplemented with 8% xylose. a TLC analysis of MEL production by the PaEMT1-complemented strain. ∆PaEMT1::neo: ∆PaEMT1 strain harboring pUXV1_neo, ∆PaEMT1::neo_PaEMT1: ∆PaEMT1 strain harboring pUXV1_neo-PaEMT1. b Cell growth (white) and PaE activity (gray). c SDS-PAGE and PaE detection through western blot analysis of culture supernatant. M, marker; P, purified PaE; Lane 1, ∆PaEMT1::neo; Lane 2, ∆PaEMT1::PaEMT1. The amount of each culture supernatant loaded in the gel was 10 μL for CBB staining and western blotting. The results of the cell growth and PaE activity assays are shown as the average of three different experiments. Error bars show standard deviations

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