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Fig. 2 | AMB Express

Fig. 2

From: Deficiency of biodegradable plastic-degrading enzyme production in a gene-deletion mutant of phyllosphere yeast, Pseudozyma antarctica defective in mannosylerythritol lipid biosynthesis

Fig. 2

Time course of PaE production by strain ∆PaEMT1 in m-3×FMM (fungal minimum medium) supplemented with 8% xylose. a Cell growth of wild-type (closed diamonds) and ∆PaEMT1 (open diamonds). b Plastic-degrading enzyme (PaE) activity of wild-type (closed diamonds) and ∆PaEMT1 (open diamonds). c sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and PaE detection through western blot analysis of culture supernatants of wild-type and ∆PaEMT1. M, marker; P, purified PaE loaded as a standard. The amount of each culture supernatant loaded in the gel was 10 μL for Coomassie brilliant blue (CBB) staining and western blotting. The results of the cell growth and PaE activity assays are shown as the average of three different experiments. Error bars show standard deviations

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