Fig. 3From: Optimization of the process parameters for reduction of gossypol levels in cottonseed meal by functional recombinant NADPH-cytochrome P450 reductase and cytochrome P450 CYP9A12 of Helicoverpa armigeraExtracted LC–MS ion chromatogram of the gossypol metabolites in negative ion mode and representative mass spectra of respective of chromatograms for quantification of gossypol and metabolites. a The control group without enzyme (blue) the free gossypol spontaneously degraded to compounds G1 (m/z 265) and G2 (m/z 293); b the recombinant of H. armigera CPR and CYP9A12 enzyme (black) was capable to degrade free gossypol by decarboxylation of G1 (m/z 265) and G2 (m/z 293) to compound G0 (m/z 209) and G0′ (m/z 249). The endogenous enzyme was as shown in blue (Chen et al. 2019)Back to article page