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Fig. 3 | AMB Express

Fig. 3

From: Optimization of the process parameters for reduction of gossypol levels in cottonseed meal by functional recombinant NADPH-cytochrome P450 reductase and cytochrome P450 CYP9A12 of Helicoverpa armigera

Fig. 3

Extracted LC–MS ion chromatogram of the gossypol metabolites in negative ion mode and representative mass spectra of respective of chromatograms for quantification of gossypol and metabolites. a The control group without enzyme (blue) the free gossypol spontaneously degraded to compounds G1 (m/z 265) and G2 (m/z 293); b the recombinant of H. armigera CPR and CYP9A12 enzyme (black) was capable to degrade free gossypol by decarboxylation of G1 (m/z 265) and G2 (m/z 293) to compound G0 (m/z 209) and G0′ (m/z 249). The endogenous enzyme was as shown in blue (Chen et al. 2019)

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