TY - JOUR AU - Shen, Yongrui AU - Li, Jianwei AU - Xiang, Junliang AU - Wang, Jiaqi AU - Yin, Kuide AU - Liu, Quan PY - 2019 DA - 2019/07/27 TI - Isolation and identification of a novel protein elicitor from a Bacillus subtilis strain BU412 JO - AMB Express SP - 117 VL - 9 IS - 1 AB - Here, we report a novel protein elicitor from Bacillus subtilis BU412 which could cause hypersensitive response (HR) and systemic acquired resistance (SAR) in tobacco. The purification was executed by ion-exchange and size exclusion chromatography. The target band on SDS-PAGE was analyzed by mass spectrometry, and the peptide mass fingerprinting matched an uncharacterized protein (WP_017418614.1), which was then named AMEP412. AMEP412 could cause a clearly defined HR necrosis in tobacco leaves, which was less affected by thermal treatment. The sub-cellular localization assay revealed that AMEP412 localized on the cell surface. This protein could also trigger early defense events such as the generation of reactive oxygen species (H2O2 and O2−) and the induction of defense enzymes, including superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL). Moreover, AMEP412 could stimulate plant systemic resistance against Pseudomonas syringae pv. tomato DC3000. SN - 2191-0855 UR - https://doi.org/10.1186/s13568-019-0822-5 DO - 10.1186/s13568-019-0822-5 ID - Shen2019 ER -