Optimization of whole-cell biotransformation for scale-up production of α-arbutin from hydroquinone by the use of recombinant Escherichia coli

AMB Express

Table 1 Related parameters in the scale-up biotransformation process of 5000-L reactor

Parameters	Batch 1#	Batch 2#
Final concentration, mM
α-Arbutin	397	375
Hydroquinone	4.54	6.18
Total yield of α-arbutin, kg	~ 378	~ 408
Total feeding amount of Hydroquinone, kg	161	175
Total amount of sucrose, kg	1150	1600
Final volume, kL	~ 3.5	~ 4.0
Catalytic time, h	22	22.5
Productivity, kg·kL⁻¹ h⁻¹	4.91	4.53
Molar conversion rate, %	95%	94.2%