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Fig. 3 | AMB Express

Fig. 3

From: Optimization of whole-cell biotransformation for scale-up production of α-arbutin from hydroquinone by the use of recombinant Escherichia coli

Fig. 3

Optimal conditions of whole-cells biotransformation for synthesis of α-arbutin. a Optimal pH. 50 mM of sodium acetate buffer (pH 3.9–6.5) (square), 50 mM of PBS (pH 5.6–7.8) (circle), and 10 mM of H3BO3-NaOH-KCl buffer (pH 7.6–10.0) (up triangle). b Optimal temperature. c Thermostability at 20 °C (square), 25 °C (circle), 30 °C (up triangle), 35 °C (down triangle), 40 °C (diamond), and 45 °C (left triangle). d Different initial concentration of hydroquinone like (mM) 20 (square), 40 (circle), 50 (up triangle), 80 (down triangle), 100 (diamond), 120 (left triangle). e The inhibitory effect of different initial concentration of fructose (black) and glucose (gray). f Sucrose hydrolysis activity by 5.0 (close) and 10.0 (open) of cell density (OD600) without addition of hydroquinone. Square: sucrose, circle: glucose, triangle: fructose

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