Table 1 Primers used in this study
Primer name | Sequence (5′-3′) | Purpose |
|---|---|---|
hph-1 | ATGAAAAAGCCTGAACTCACCGCGACG | Probe in Southern blot and genomic PCR for pCsGi-hph |
hph-2 | CTATTCCTTTGCCCTCGGACGAGTGCT | Probe in Southern blot and genomic PCR for pCsGi-hph |
Csbar_F | TCAGGTGCGTGAAGTGTACT | Probe in Southern blot and genomic PCR for pCsbtubi-hph |
hph-4_R | TACGTAGGTAGTGGTAGACT | Probe in Southern blot and genomic PCR for pCsbtubi-hph |
CsGPD-TATAmut_R | GAGGGTTGATATGGGGCGAT | Site-directed mutagenesis for p201[A60C] |
CsGPD-8 | ACCCCGCCGCTTTTTCCCTTCAT | Site-directed mutagenesis for p201[A60C] |
CsGP-PRO5 | CGCCAAGCTTACTCATCCAGAATACACTCG | PCR amplification of Csgpd promoter, and the first exon and intron |
CsGP-PRO3int | CTGCAATTGTCCGTCAGTAC | PCR amplification of Csgpd promoter, and the first exon and intron |
Csbtub-pro-R1 | GCTAGAGACGATATCAGTT | PCR amplification of Csbtub promoter |
Csbtub-Hind_F2 | AGGCTCTAAGCTTGTACCGAGTGCAT | PCR amplification of Csbtub promoter |