Fig. 1

Schematic of pCsGi-hph and pCsbtubi-hph plasmids. Plasmids pCsGi-hph and pCsbtubi-hph were constructed by combining the homologous promoter region of gpd and beta-tubulin genes from C. subvermispora, respectively, with the coding sequence of bacterial hph. In pCsGi-hph, the first exon, ATGCCC, and the first intron from Csgpd were inserted just before the hygromycin phosphotransferase sequence. In pCsbtubi-hph, the first and second introns, as well as the first exon (encoding 11 amino acids), from Csß-tub were inserted just before the hygromycin phosphotransferase sequence. The plasmid backbones of pCsGi-hph and pCsbtubi-hph are pCRII (Thermo Fisher Scientific Inc.) and pUC19 (Takara Bio Inc.), respectively