Fig. 3

SDS-PAGE analysis for confirmation of BacD expression. The supernatant (7 μg of the total protein) of cell lysate in WT pQE85 pCysHP and WT pQE85-BsBacD pCysHP was analyzed by SDS-PAGE. The protein band considered to be expressed BacD was shown by black arrow. The cell cultures for 16 h with Terrific Broth media were performed adding different IPGT concentrations (0, 0.05, 0.1, 1, and 5 mM [final concentrations]) at 3 h and it is indicated above. Protein marker size (kDa) was indicated above. For details, see “Materials and methods”