Fig. 2

The crude extracts of red centipede’s haemolymph, gut and muscles were prepared and tested in antibacterial bioassays. For negative control, bacteria incubated with nutrient broth/PBS was used and for positive control bacteria incubated with 100 μg/mL of gentamicin was used. Asterisk represents P < 0.05. P values were obtained using two-sample T test and two-tailed distribution. a Represents 0% growth indicating potent bacteriostatic activity of 10% haemolymph, 100 μg/mL of muscle and gut extracts of red centipede against MRSA. b Represents cidal assay, indicating 50%, 80% and 68% viability of respective extracts against MRSA. c Represents more than 90% bacteriostatic activity of all the three extracts against B. cereus. d Also represents more than 90% bactericidal activity for all three extracts against B. cereus. e Represents 49%, 31% and 63% growth in bacteriostatic assays respectively against K. pneumoniae. f Represents 48%, 53% and 75% viability in bactericidal assays respectively against K. pneumoniae. g Represents 22%, 10% and 49% growth in bacteriostatic assays respectively against S. enterica. h Represents 55% and 78% viability for haemolymh and gut extracts however, muscle extracts was not active in bactericidal assays against S. enterica. i Represents 27%, 31% and 70% growth in bacteriostatic assays respectively against E. coli K1. j Represents 49%, 44% and 73% viability in bactericidal assays respectively against E. coli K1. k Represents nearly 50% bacteriostatic activity of all three extracts against S. marcescens. l Represents no bactericidal activity of centipede’s extracts against S. marcescens. m Represents nearly 83, 81 and 47% growth of centipede’s haemolymph, gut and muscles respectively against P. aeruginosa. n Represents no bactericidal activity of centipede’s extracts against P. aeruginosa. o Represents nearly 33, 19 and 38% growth of centipede’s haemolymph, gut and muscles respectively against S. pyogenes. p Represents 63, 50 and 51% viability of the extracts respectively against S. pyogenes. The results are representative of several experiments performed in duplicates and expressed as the mean ± standard error