Rumen-protected methionine a feed supplement to low dietary protein: effects on microbial population, gases production and fermentation characteristics

AMB Express

Table 4 Addition of RPMet with low CP their effects on daily production of total and individual volatile fatty acids using the RUSITEC (n = 4)

Items	Treatment				SEM	P-value
Items	HP	LP	LPLMet	LPHMet	SEM	P-value
Total VFA (mM)	100.17^a	87.42^c	91.33^b	100.47^a	1.71	< 0.01
Acetate (mM)	50.74^a	45.17^b	47.17^b	47.78^ab	0.35	< 0.01
Propionate (mM)	27.60^a	25.50^b	25.83^b	27.50^a	0.32	< 0.01
Isobutyrate (mM)	0.83	0.81	0.82	0.83	0.36	0.99
Butyrate (mM)	13.67^a	10.33^b	11.07^b	12.91^a	0.45	< 0.01
Isovalerate (mM)	2.97	3.03	3.06	3.07	0.18	0.99
Valerate (mM)	4.18	3.65	3.89	4.09	0.02	0.79
Acetate: propionate ratio	1.84^ab	1.75^b	1.82^ab	1.87^a	0.02	0.04

VFA, volatile fatty acids; SEM, standard error of mean
^a,b,cSuperscripts values within the same row, are significantly different at (P < 0.05)