Fig. 4

a Effect of NaCl on the activity of XylCMS. Activity assays were conducted in various NaCl concentrations in citrate buffer (pH 6) at 37 °C and 55 °C. The enzyme activities are shown as relative (%) to the enzyme activity in salt-free condition at 55 °C. b Thermal stability of XylCMS during 50 min incubation at 50 °C, 55 °C, and 60 °C in citrate buffer (pH 6). The residual activity was determined at 10 min intervals in citrate buffer (pH 6) at 55 °C and is shown as relative (%) to the enzyme activity before incubation. c Thermal stability of XylCMS under the influence of salt during 50 min incubation at 50 °C, 55 °C, and 60 °C in citrate buffer (pH 6) containing 3 M NaCl. The residual activity was determined at 10 min intervals in citrate buffer (pH 6) at 55 °C and is shown as relative (%) to the enzyme activity before incubation. d The catalytic activity of XylCMS in artificial seawater compared with that in the salt-free control condition and 3 M NaCl at 37 °C and 55 °C. The activities are presented as relative (%) to the enzyme activity in the salt-free condition at 55 °C