Fig. 3

a Effect of pH on the activity of XylCMS. Activity assays were conducted in various pH buffers including citrate buffer for pH 3–6, phosphate buffer for pH 6–8, and glycine–NaOH buffer for pH 8–10 at 37 °C. The enzyme activities are shown as relative (%) to the highest value obtained at pH 6. b Effect of temperature on the activity of XylCMS. Activity assays were performed in citrate buffer (pH 6) at various temperatures under salt-free standard and 3 M NaCl conditions. The enzyme activities are shown as relative (%) to the highest values obtained at 55 °C