Table 3 Sensitivity and robustness of fluridone detection method
From: Development of extraction and detection method for fluridone in water and sediment by HPLC-UV
Parameters | Change in parameter values | (min) | Peak area counts | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Run 1 | Run 2 | Run 3 | Avg. | Std. dev. | Run 1 | Run 2 | Run 3 | Avg. | Std. dev. | ||
Flow (mL/min) | 1 | 4.28 | 4.33 | 4.33 | 4.31 | 0.03 | 92,311 | 92,398.00 | 92,005.00 | 92,238.00 | 206.42 |
0.8 | 5.41 | 5.41 | 5.41 | 5.41 | 0.00 | 115,100.00 | 115,346.00 | 114,780.00 | 115,075.33 | 283.81 | |
1.2 | 3.65 | 3.64 | 3.64 | 3.64 | 0.01 | 76,440.00 | 77,136.00 | 76,539.00 | 76,705.00 | 376.52 | |
Temperature (°C) | 28 | 4.29 | 4.29 | 4.29 | 4.29 | 0.00 | 92,979.00 | 93,011.00 | 93,520.00 | 93,170.00 | 303.53 |
26 | 4.32 | 4.33 | 4.33 | 4.33 | 0.01 | 93,687.00 | 90,813.00 | 91,629.00 | 92,043.00 | 1481.05 | |
30 | 4.26 | 4.25 | 4.26 | 4.26 | 0.01 | 92,054.00 | 92,344.00 | 93,267.00 | 92,555.00 | 633.43 | |
Mobile phase (Acetonitrile:H2O) (%) | 50:50 | 8.25 | 8.27 | 8.27 | 8.26 | 0.01 | 93,517.00 | 92,177.00 | 93,384.00 | 93,026.00 | 738.26 |
70:30 | 2.94 | 2.83 | 2.83 | 2.87 | 0.06 | 90,582.00 | 91,803.00 | 93,268.00 | 91,884.33 | 1344.85 | |
80:20 | 2.17 | 2.11 | 2.11 | 2.13 | 0.03 | 90,124.00 | 91,063.00 | 90,963.00 | 90,716.67 | 515.69 | |