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Table 2 Results of β-galactosidase activity (U/mL), according to the 23 factorial design with centered face and three repetitions at the central point central

From: Optimization and partial purification of beta-galactosidase production by Aspergillus niger isolated from Brazilian soils using soybean residue

Run orders Coded levels β-Galactosidaseactivity (U/mL)a
X 1 X 2 X 3
1 − 1 − 1 − 1 19.64
2 1 − 1 − 1 19.02
3 − 1 1 − 1 7.07
4 1 1 − 1 8.83
5 − 1 − 1 1 10.06
6 1 − 1 1 0.03
7 − 1 1 1 0.00
8 1 1 1 1.12
9 0 0 0 23.4
10 0 0 0 24.64
11 0 0 0 22.60
  1. Data: (X1) speed agitation, (X2) temperature and (X3) pH on β-galactosidase production
  2. aOne unit (U) of β-galactosidase activity was defined as the amount of the enzyme catalyzing the release of 1 µmol of o-nitrophenol per min at 410 nm (Nagy et al. 2001)