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Table 1 Summary of cultivation parameters

From: Heterologous rhamnolipid biosynthesis by P. putida KT2440 on bio-oil derived small organic acids and fractions

Carbon source (CS) cCS (g/L) Growth cBMmax (g/L) µmax (h−1) YX|Smax (g/g) YP|X (g/g) cRLmax (mg/L) qRLmax (mg/g/h) qRLavg (mg/g/h) References
Sunflower oil
 P. aeruginosa wt, different strains Excess 9.0–19.0 0.09–0.18 n.d. 0.9–3.1 8.0–38.0 × 103 60–190 10–40 Müller et al. (2011)
Glucose
 P. aeruginosa wt, different strains 20–40 2.4–7.7 n.d. n.d. 0.3–2.3 0.2–6.1 × 103 n.d. 12–54 Henkel et al. (2016)
 P. putida KT2440 pSynpro8oT_rhlAB Excess 23.6 0.44 0.24–0.33 n.d. 14.9 × 103 n.d. 18–24 Beuker et al. (2016a, b)
 P. putida KT2440 pSynpro8oT_rhlAB 10 2.67 0.38 0.26 0.61 827.7 71.9 42.4 This study
Small organic acids
 Acetate (A) 1 0.20 0.38 0.24 0.76 88.5 74.8 52.7 This study
3 0.63 0.52 0.23 0.57 191.2 66.7 58.6
5 0.80 0.40 0.21 0.62 264.5 62.4 39.2
10 0.70 0.15 0.12 0.72 264.2 43.7 23.5
 Formate (F) 1 0 0 n.d. 0 0 0 0
3 0 0 n.d. 0 0 0 0
5 0 0 n.d. 0 0 0 0
 Propionate (P) 1 0.23 0.25 n.d. 0.90 119.4 74.7 62.4
3 0.73 0.24 n.d. 0.47 184.0 49.8 53.9
5 0.67 0.16 n.d. 0.61 212.3 19.9 24.7
10 2.57 0.21 n.d. 0.07 93.7 n.a. n.a.
Mixtures of small organic acids
 AF 5 + 1 0.63 0.49 0.18 0.56 185.9 56.9 40.4
 AP 5 + 1 0.87 0.50 0.21 0.53 239.7 80.6 40.8
 AFP 5 + 1 + 1 0.90 0.37 0.25 0.41 193.9 72.1 26.6
Bio-oil fractions
 OCSPE 1a 0.27 0.39 0.24 0.48 72.5 28.4 n.a.
3a 0.37 0.20 0.10 0.43 89.4 2.8 1.8
5a 0 0 n.a. 0 0 0 0
 ACSPE 1a 0 0 n.a. 0 0 0 0
3a 0 0 n.a. 0 0 0 0
5a 0 0 n.a. 0 0 0 0
  1. aThe bio-oil fractions were added in three different concentrations adjusted according to acetate concentration
  2. ✔ growth, ✖ no growth observed, n.a. not applicable, n.d. not determined