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Table 1 Summary of cultivation parameters

From: Heterologous rhamnolipid biosynthesis by P. putida KT2440 on bio-oil derived small organic acids and fractions

Carbon source (CS)

cCS (g/L)

Growth

cBMmax (g/L)

µmax (h−1)

YX|Smax (g/g)

YP|X (g/g)

cRLmax (mg/L)

qRLmax (mg/g/h)

qRLavg (mg/g/h)

References

Sunflower oil

 P. aeruginosa wt, different strains

Excess

9.0–19.0

0.09–0.18

n.d.

0.9–3.1

8.0–38.0 × 103

60–190

10–40

Müller et al. (2011)

Glucose

 P. aeruginosa wt, different strains

20–40

2.4–7.7

n.d.

n.d.

0.3–2.3

0.2–6.1 × 103

n.d.

12–54

Henkel et al. (2016)

 P. putida KT2440 pSynpro8oT_rhlAB

Excess

23.6

0.44

0.24–0.33

n.d.

14.9 × 103

n.d.

18–24

Beuker et al. (2016a, b)

 P. putida KT2440 pSynpro8oT_rhlAB

10

2.67

0.38

0.26

0.61

827.7

71.9

42.4

This study

Small organic acids

 Acetate (A)

1

0.20

0.38

0.24

0.76

88.5

74.8

52.7

This study

3

0.63

0.52

0.23

0.57

191.2

66.7

58.6

5

0.80

0.40

0.21

0.62

264.5

62.4

39.2

10

0.70

0.15

0.12

0.72

264.2

43.7

23.5

 Formate (F)

1

0

0

n.d.

0

0

0

0

3

0

0

n.d.

0

0

0

0

5

0

0

n.d.

0

0

0

0

 Propionate (P)

1

0.23

0.25

n.d.

0.90

119.4

74.7

62.4

3

0.73

0.24

n.d.

0.47

184.0

49.8

53.9

5

0.67

0.16

n.d.

0.61

212.3

19.9

24.7

10

2.57

0.21

n.d.

0.07

93.7

n.a.

n.a.

Mixtures of small organic acids

 AF

5 + 1

0.63

0.49

0.18

0.56

185.9

56.9

40.4

 AP

5 + 1

0.87

0.50

0.21

0.53

239.7

80.6

40.8

 AFP

5 + 1 + 1

0.90

0.37

0.25

0.41

193.9

72.1

26.6

Bio-oil fractions

 OCSPE

1a

0.27

0.39

0.24

0.48

72.5

28.4

n.a.

3a

0.37

0.20

0.10

0.43

89.4

2.8

1.8

5a

0

0

n.a.

0

0

0

0

 ACSPE

1a

0

0

n.a.

0

0

0

0

3a

0

0

n.a.

0

0

0

0

5a

0

0

n.a.

0

0

0

0

  1. aThe bio-oil fractions were added in three different concentrations adjusted according to acetate concentration
  2. ✔ growth, ✖ no growth observed, n.a. not applicable, n.d. not determined