Fig. 7
From: Isolation and identification of a TetR family protein that regulates the biodesulfurization operon
a A 12% SDS-PAGE gel showing expression of Dsz proteins (DszA ~ 52 kDa, DszC ~ 45 kDa) in presence of the TetR family protein when the cells are grown in DBT containing medium. Lane1: marker, lane2: Wild type Gordonia (sonicated supernatant), lane3: Gordonia transformed with the TetR family protein uninduced (sonicated supernatant), lane4: Gordonia transformed with the TetR family protein induced (sonicated supernatant). The proteins were identified by MALDI-ToF. b A 12% SDS-PAGE gel showing the expression of Dsz proteins when cells are grown in DBT containing medium. Lane1: marker, lane2: Wild type Gordonia (sonicated supernatant) when induced with IPTG at Day 0, lane 3: Wild type Gordonia (sonicated supernatant) when induced at OD600 is 0.5. Induction of the dsz enzymes are not observed in absence of the TetR family protein