Fig. 2
From: Cell-free enzymatic synthesis of GDP-l-fucose from mannose
a SDS-PAGE analysis of the recombinant GDP-l-fucose biosynthetic enzymes. Lane M: molecular weight marker; Lane 1–3: Glk (−IPTG), Glk (+IPTG), purified Glk; Lane 4–6: ManB (−IPTG), ManB (+IPTG), purified ManB; Lane 7–9: ManC (−IPTG), ManC (+IPTG), purified ManC; Lane 10–12: Gmd (−IPTG), Gmd (+IPTG), purified Gmd; Lane 13–15: WcaG (−IPTG), WcaG (+IPTG), purified WcaG. b Protein concentrations of recombinant enzymes