Comprehensive investigation on the synergistic antibacterial activities of Jatropha curcas pressed cake and seed oil in combination with antibiotics

Haq, Abdul; Siddiqi, Maleeha; Batool, Syeda Zakia; Islam, Arshad; Khan, Alam; Khan, Dildar; Khan, Samiullah; Khan, Haji; Shah, Aamer Ali; Hasan, Fariha; Ahmed, Safia; Badshah, Malik

doi:10.1186/s13568-019-0793-6

AMB Express

Table 8 Minimum inhibitory concentration (MIC) and fractional inhibitory concentration (FIC) of seed oil, each antibiotic alone and their combination against MDR pathogenic bacterial isolates

From: Comprehensive investigation on the synergistic antibacterial activities of Jatropha curcas pressed cake and seed oil in combination with antibiotics

Strain		MIC^a (mg/mL)/MIC^b (µg/mL)		FIC	FICI	Output
Strain	Compound	Alone	Combined	FIC	FICI	Output
MRSA1	Oil/Ctx	50/100	100/50	2/0.5	2.5	Indifference
	Oil/R	50/100	100/50	–/0.5	ND	ND
	Oil/Of	50/100	3.125/1.56	0.06/0.01	0.07	Synergistic
	Oil/Cip	50/50	100/50	–/1	ND	ND
	Oil/Mox	50/1.56	25/12.5	–/8.01	ND	ND
MRSA2	Oil/Ctx	–/100	25/12.5	–/0.1	ND	ND
	Oil/R	–/3.125	25/12.5	–/4	ND	ND
	Oil/Of	–/25	1.56/0.78	–/0.06	ND	ND
	Oil/Cip	–/50	1.56/0.78	–/0.03	ND	ND
	Oil/Mox	–/0.781	6.25/3.125	–/4	ND	ND
MRSA3	Oil/Ctx	–/50	3.125/1.56	–/0.03	ND	ND
	Oil/R	–/3.125	3.125/1.56	–/0.03	ND	ND
	Oil/Of	–/50	50/25	–/0.0078	ND	ND
	Oil/Cip	–/12.5	25/12.5	–/0.03	ND	ND
	Oil/Mox	–/0.39	3.125/1.56	–/8.01	ND	ND
MRSA4	Oil/Ctx	–/50	0.39/0.195	–/0.003	ND	ND
	Oil/R	–/3.125	3.125/1.56	–/0.49	ND	ND
	Oil/Of	–/1.56	1.56/0.78	–/0.5	ND	ND
	Oil/Cip	–/3.125	3.125/1.56	–/0.49	ND	ND
	Oil/Mox	–/0.781	100/50	–/1.99	ND	ND
MRSA5	Oil/Ctx	–/3.125	50/25	–/8	ND	ND
	Oil/R	–/3.125	0.78/0.39	–/0.12	ND	ND
	Oil/Of	–/100	12.5/6.25	–/0.06	ND	ND
	Oil/Cip	–/50	100/50	–/1	ND	ND
	Oil/Mox	–/1.56	6.25/3.125	–/32	ND	ND
A. baumannii MDR	Oil/Ctx	–/6.25	3.125/1.56	–/0.24	ND	ND
	Oil/R	–/3.125	0.195/0.097	–/0.03	ND	ND
	Oil/Of	–/0.78	0.78/0.3	–/0.5	ND	ND
	Oil/Cip	–/6.25	0.78/0.39	–/0.06	ND	ND
	Oil/Mox	–/1.56	3.125/1.56	–/1	ND	ND
K. pneumoniae	Oil/Ctx	–/100	100/50	–/0.5	ND	ND
	Oil/R	–/100	100/50	–/0.5	ND	ND
	Oil/Of	–/25	100/50	–/2	ND	ND
	Oil/Cip	–/50	12.5/6.25	–/0.12	ND	ND
	Oil/Mox	–/1.56	6.25/3.125	–/2	ND	ND
P. chlororaphis	Oil/Ctx	–/–	–/–	–/–	ND	ND
	Oil/R	–/100	1.56/0.78	–/0.0078	ND	ND
	Oil/Of	–/6.25	100/50	–/8	ND	ND
	Oil/Cip	–/3.125	3.125/1.56	–/0.4	ND	ND
	Oil/Mox	–/1.56	6.253.125	–/2	ND	ND
P. monteilii	Oil/Ctx	–/100	6.25/3.125	–/0.03	ND	ND
	Oil/R	–/–	0.78/0.39	–/–	ND	ND
	Oil/Of	–/100	0.78/0.39	–/0.0039	ND	ND
	Oil/Cip	–/50	0.78/0.39	–/0.0078	ND	ND
	Oil/Mox	–/0.781	100/50	–/64	ND	ND

MIC^a minimal inhibitory concentration for seed oil when applied alone, MIC^b minimal inhibitory concentration for antibiotic when applied alone, Me methanolic extract, Ctx cefotaxime, R rifampicin, Of ofloxacin, Cip ciprofloxacin, Mox moxifloxacin, ND not determined

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