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Table 5 Minimum inhibitory concentration (MIC) and fractional inhibitory concentration (FIC) of methanolic extract, each antibiotic alone and their combination against MDR pathogenic bacterial isolates

From: Comprehensive investigation on the synergistic antibacterial activities of Jatropha curcas pressed cake and seed oil in combination with antibiotics

Strain Compound MICa (mg/mL)/MICb (µg/mL) FIC FICI Output
Alone Combined
MRSA1 Me/Ctx 50/100 50/25 0.5/2 2.5 Indifference
Me/R 50/100 100/50 0.5/2 2.5 Indifference
Me/Of 50/100 100/50 0.5/2 2.5 Indifference
Me/Cip 50/50 25/12.5 0.5/0.25 0.75 Additive
Me/Mox 50/1.56 25/12.5 0.5/8.01 8.51 Antagonistic
MRSA2 Me/Ctx 50/100 50/25 1/4 5.0 Antagonistic
Me/R 50/3.125 1.56/0.78 0.03/0.24 0.28 Synergistic
Me/Of 50/25 100/50 2/2 4.0 Indifference
Me/Cip 50/50 50/25 1/0.5 1.5 Indifference
Me/Mox 50/0.781 12.5/6.25 0.25/8 8.25 Antagonistic
MRSA3 Me/Ctx 50/50 50/25 1/0.5 1.5 Indifference
Me/R 50/3.125 0.19/0.09 0.003/0.006 0.01 Synergistic
Me/Of 50/50 50/25 1/0.5 1.5 Indifference
Me/Cip 50/12.5 25/12.5 0.5/1 1.5 Indifference
Me/Mox 50/0.39 3.125/1.56 0.06/4 4.06 Antagonistic
MRSA4 Me/Ctx 100/50 1.56/0.78 0.01/0.01 0.03 Synergistic
Me/R 100/3.125 0.78/0.39 0.007/0.12 0.13 Synergistic
Me/Of 100/1.56 0.78/0.78 0.007/0.5 0.5 Synergistic
Me/Cip 100/3.125 1.56/0.39 0.01/0.12 0.14 Synergistic
Me/Mox 100/0.781 25/12.5 0.25/16 16.25 Antagonistic
MRSA5 Me/Ctx 100/3.125 100/50 1/16 17 Antagonistic
Me/R 100/3.125 0.78/0.39 0.007/0.12 0.13 Synergistic
Me/Of 100/100 50/25 0.5/4 4.5 Antagonistic
Me/Cip 100/50 50/25 0.5/0.5 1.0 Indifference
Me/Mox 100/1.56 6.25/3.125 0.06/2 2.06 Indifference
A. baumannii MDR Me/Ctx 100/6.25 3.12/1.56 0.03/0.24 0.2 Synergistic
Me/R 100/3.125 0.39/0.195 0.003/0.06 0.06 Synergistic
Me/Of 100/0.78 1.56/0.78 0.01/1 1.01 Indifference
Me/Cip 100/6.25 0.39/0.195 0.003/0.03 0.03 Synergistic
Me/Mox 100/1.56 6.25/3.125 0.06/2 2.0 Indifference
K. pneumoniae Me/Ctx 100/100 100/50 1/2 3.0 Indifference
Me/R 100/100 50/25 0.5/0.25 0.75 Additive
Me/Of 100/25 50/25 0.5/0.03 0.5 Synergistic
Me/Cip 100/50 50/25 0.5/0.003 0.5 Synergistic
Me/Mox 100/1.56 12.5/6.25 0.12/4 4.13 Antagonistic
P. chlororaphis Me/Ctx 100/– 25/12.5 0.25/– ND ND
Me/R 100/100 25/12.5 0.25/0.125 0.38 Synergistic
Me/Of 100/6.25 100/50 1/0.12 1.12 Indifference
Me/Cip 100/3.125 12.5/6.25 0.12/0.06 0.18 Synergistic
Me/Mox 100/1.56 3.125/1.56 0.03/1 1.03 Indifference
P. monteilii Me/Ctx –/100 0.39/0.195 –/512 ND ND
Me/R –/– 0.39/0.195 –/– ND ND
Me/Of –/100 0.78/0.39 –/256 ND ND
Me/Cip –/50 0.39/0.195 –/0.003 ND ND
Me/Mox –/0.78 100/50 –/64 ND ND
  1. MICa minimal inhibitory concentration for de-oiled seed extract when applied alone, MICb minimal inhibitory concentration for antibiotic when applied alone, Me methanolic extract, Ctx cefotaxime, R rifampicin, Of ofloxacin, Cip ciprofloxacin, Mox moxifloxacin, ND not determined