Table 4 Minimum inhibitory concentration (MIC) and fractional inhibitory concentration (FIC) of seed oil each antibiotic and their combination against clinical pathogenic and reference bacterial isolates
Strain | Compound | MICa (mg/mL)/MICb (µg/mL) | FIC | FICI | Output | |
|---|---|---|---|---|---|---|
Alone | Combined | |||||
A. baumannii | Oil/Ctx | 100/0.19 | 0.39/0.195 | 0.0039/1.026 | 1.03 | Synergistic |
Oil/R | 100/6.25 | 0.78/0.39 | 0.0078/0.062 | 0.07 | Antagonistic | |
Oil/Of | 100/12.5 | 6.25/3.125 | 0.25/0.25 | 0.5 | Synergistic | |
Oil/Cip | 100/6.25 | 6.25/3.125 | 0.031/0.5 | 0.5 | Synergistic | |
Oil/Mox | 100/6.25 | 50/25 | 0.5/2 | 4.5 | Antagonistic | |
E. coli | Oil/Ctx | 200/100 | 6.25/3.125 | 0.031/0.031 | 0.06 | Synergistic |
Oil/R | 200/100 | 0.78/0.39 | 0.0039/0.0039 | 0.0078 | Synergistic | |
Oil/Of | 200/50 | 0.78/12.5 | 0.125/0.25 | 0.3 | Synergistic | |
Oil/Cip | 200/100 | 25/0.195 | 0.0078/0.0039 | 0.01 | Synergistic | |
Oil/Mox | 200/3.125 | 12.5/6.25 | 0.125/2 | 2.12 | Indifference | |
E. faecalis | Oil/Ctx | 100/50 | 1.56/0.78 | 0.015/0.015 | 0.03 | Synergistic |
Oil/R | 100/100 | 1.56/0.78 | 0.015/0.0078 | 0.02 | Synergistic | |
Oil/Of | 100/12.5 | 1.56/1.56 | 0.015/0.124 | 0.1 | Synergistic | |
Oil/Cip | 100/50 | 1.56/0.78 | 0.015/0.015 | 0.03 | Synergistic | |
Oil/Mox | 100/1.56 | 1.56/3.125 | 0.06/2 | 2.0 | Indifference | |
P. vulgaris | Oil/Ctx | 100/0.19 | 0.39/0.195 | 0.0039/0.03 | 0.03 | Synergistic |
Oil/R | 100/6.25 | 3.125/1.56 | 0.03/0.24 | 0.2 | Synergistic | |
Oil/Of | 100/12.5 | 3.125/0.195 | 0.03/0.015 | 0.04 | Synergistic | |
Oil/Cip | 100/6.25 | 0.78/1.56 | –/0.24 | ND | ND | |
Oil/Mox | 100/6.25 | 100/50 | –/16 | ND | ND | |
S. aureus | Oil/Ctx | –/50 | 3.125/1.56 | –/0.03 | ND | ND |
Oil/R | –/6.25 | 1.56/0.78 | –/0.12 | ND | ND | |
Oil/Of | –/100 | 1.56/0.78 | –/0.06 | ND | ND | |
Oil/Cip | –/12.5 | 0.195/0.097 | 0.0009/0.0077 | 0.008 | Synergistic | |
Oil/Mox | –/3.125 | 12.5/6.25 | 0.03/2 | 2.0 | Indifference | |
P. aeruginosa | Oil/Ctx | 200/50 | 0.39/0.195 | 0.001/0.0039 | 0.005 | Synergistic |
Oil/R | 200/6.25 | 12.5/6.25 | 0.06/1 | 1.0 | Indifference | |
Oil/Of | 200/12.5 | 6.25/3.125 | 0.03/0.1 | 0.1 | Synergistic | |
Oil/Cip | 200/3.125 | 3.125/3.125 | 2/1 | 3.0 | Indifference | |
Oil/Mox | 200/0.39 | 3.125/1.56 | 0.25/4 | 4.2 | Antagonistic | |
S. aureus(ATCC) | Oil/Ctx | –/100 | 0.78/0.39 | –/0.003 | ND | ND |
Oil/R | –/6.25 | 0.195/0.097 | –/0.01 | ND | ND | |
Oil/Of | –/3.125 | 0.78/0.39 | –/0.12 | ND | ND | |
Oil/Cip | –/1.56 | 0.195/0.0485 | –/0.03 | ND | ND | |
Oil/Mox | –/0.781 | 3.125/1.56 | –/1.99 | ND | ND | |
E. coli (ATCC) | Oil/Ctx | 100/0.781 | 0.78/0.39 | 0.0078/0.5 | 0.5 | Synergistic |
Oil/R | 100/6.25 | 0.78/0.39 | 0.0078/0.06 | 0.07 | Synergistic | |
Oil/Of | 100/3.125 | 0.095/0.0485 | 0.00097/0.015 | 0.01 | Synergistic | |
Oil/Cip | 100/3.125 | 0.095/0.0485 | 0.0097/0.01 | 0.01 | Synergistic | |
Oil/Mox | 100/0.39 | 6.25/3.125 | 0.06/8.01 | 8.0 | Antagonistic | |