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Table 4 Minimum inhibitory concentration (MIC) and fractional inhibitory concentration (FIC) of seed oil each antibiotic and their combination against clinical pathogenic and reference bacterial isolates

From: Comprehensive investigation on the synergistic antibacterial activities of Jatropha curcas pressed cake and seed oil in combination with antibiotics

Strain Compound MICa (mg/mL)/MICb (µg/mL) FIC FICI Output
Alone Combined
A. baumannii Oil/Ctx 100/0.19 0.39/0.195 0.0039/1.026 1.03 Synergistic
Oil/R 100/6.25 0.78/0.39 0.0078/0.062 0.07 Antagonistic
Oil/Of 100/12.5 6.25/3.125 0.25/0.25 0.5 Synergistic
Oil/Cip 100/6.25 6.25/3.125 0.031/0.5 0.5 Synergistic
Oil/Mox 100/6.25 50/25 0.5/2 4.5 Antagonistic
E. coli Oil/Ctx 200/100 6.25/3.125 0.031/0.031 0.06 Synergistic
Oil/R 200/100 0.78/0.39 0.0039/0.0039 0.0078 Synergistic
Oil/Of 200/50 0.78/12.5 0.125/0.25 0.3 Synergistic
Oil/Cip 200/100 25/0.195 0.0078/0.0039 0.01 Synergistic
Oil/Mox 200/3.125 12.5/6.25 0.125/2 2.12 Indifference
E. faecalis Oil/Ctx 100/50 1.56/0.78 0.015/0.015 0.03 Synergistic
Oil/R 100/100 1.56/0.78 0.015/0.0078 0.02 Synergistic
Oil/Of 100/12.5 1.56/1.56 0.015/0.124 0.1 Synergistic
Oil/Cip 100/50 1.56/0.78 0.015/0.015 0.03 Synergistic
Oil/Mox 100/1.56 1.56/3.125 0.06/2 2.0 Indifference
P. vulgaris Oil/Ctx 100/0.19 0.39/0.195 0.0039/0.03 0.03 Synergistic
Oil/R 100/6.25 3.125/1.56 0.03/0.24 0.2 Synergistic
Oil/Of 100/12.5 3.125/0.195 0.03/0.015 0.04 Synergistic
Oil/Cip 100/6.25 0.78/1.56 –/0.24 ND ND
Oil/Mox 100/6.25 100/50 –/16 ND ND
S. aureus Oil/Ctx –/50 3.125/1.56 –/0.03 ND ND
Oil/R –/6.25 1.56/0.78 –/0.12 ND ND
Oil/Of –/100 1.56/0.78 –/0.06 ND ND
Oil/Cip –/12.5 0.195/0.097 0.0009/0.0077 0.008 Synergistic
Oil/Mox –/3.125 12.5/6.25 0.03/2 2.0 Indifference
P. aeruginosa Oil/Ctx 200/50 0.39/0.195 0.001/0.0039 0.005 Synergistic
Oil/R 200/6.25 12.5/6.25 0.06/1 1.0 Indifference
Oil/Of 200/12.5 6.25/3.125 0.03/0.1 0.1 Synergistic
Oil/Cip 200/3.125 3.125/3.125 2/1 3.0 Indifference
Oil/Mox 200/0.39 3.125/1.56 0.25/4 4.2 Antagonistic
S. aureus(ATCC) Oil/Ctx –/100 0.78/0.39 –/0.003 ND ND
Oil/R –/6.25 0.195/0.097 –/0.01 ND ND
Oil/Of –/3.125 0.78/0.39 –/0.12 ND ND
Oil/Cip –/1.56 0.195/0.0485 –/0.03 ND ND
Oil/Mox –/0.781 3.125/1.56 –/1.99 ND ND
E. coli (ATCC) Oil/Ctx 100/0.781 0.78/0.39 0.0078/0.5 0.5 Synergistic
Oil/R 100/6.25 0.78/0.39 0.0078/0.06 0.07 Synergistic
Oil/Of 100/3.125 0.095/0.0485 0.00097/0.015 0.01 Synergistic
Oil/Cip 100/3.125 0.095/0.0485 0.0097/0.01 0.01 Synergistic
Oil/Mox 100/0.39 6.25/3.125 0.06/8.01 8.0 Antagonistic
  1. MICa minimal inhibitory concentration for seed oil when applied alone, MICb minimal inhibitory concentration for antibiotic when applied alone, Me methanolic extract, Ctx cefotaxime, R rifampicin, Of ofloxacin, Cip ciprofloxacin, Mox moxifloxacin, ND not determined