Table 3 Minimum inhibitory concentration (MIC) and fractional inhibitory concentration (FIC) of aqueous extract, each antibiotic and their combination against clinical pathogenic and reference bacterial isolates
Strain | Compound | MICa (mg/mL)/MICb (µg/mL) | FIC | FICI | Output | |
|---|---|---|---|---|---|---|
Alone | Combined | |||||
A. baumannii | Aq/Ctx | 200/0.19 | 0.195/0.097 | 0.0009/0.5 | 0.5 | Synergistic |
Aq/R | 200/6.25 | 1.56/0.78 | 0.007/0.12 | 0.1 | Synergistic | |
Aq/Of | 200/12.5 | 3.125/1.56 | 0.03/0.12 | 0.1 | Synergistic | |
Aq/Cip | 200/6.25 | 1.56/0.78 | 0.007/0.12 | 0.1 | Synergistic | |
Aq/Mox | 200/6.25 | 12.5/6.25 | 0.12/1 | 1.125 | Indifference | |
E. coli | Aq/Ctx | –/100 | 100/50 | –/0.5 | ND | ND |
Aq/R | –/100 | 3.125/1.56 | –/0.01 | ND | ND | |
Aq/Of | –/50 | 6.25/3.125 | –0.06 | ND | ND | |
Aq/Cip | –/100 | 6.25/3.125 | –/0.03 | ND | ND | |
Aq/Mox | –/3.125 | 25/12.5 | –/4 | ND | ND | |
E. faecalis | Aq/Ctx | –/50 | 100/50 | –/1 | ND | ND |
Aq/R | –/100 | 1.56/0.78 | –/0.007 | ND | ND | |
Aq/Of | –/12.5 | 1.56/0.78 | –/0.06 | ND | ND | |
Aq/Cip | –/50 | 3.125/1.56 | –/0.03 | ND | ND | |
Aq/Mox | –/1.56 | 50/25 | –/16 | ND | ND | |
P. vulgaris | Aq/Ctx | –/0.19 | 6.25/3.125 | –/0.5 | ND | ND |
Aq/R | –/6.25 | 6.25/3.125 | –/0.5 | ND | ND | |
Aq/Of | –/12.5 | 0.78/0.39 | –/0.03 | ND | ND | |
Aq/Cip | –/6.25 | 0.78/0.39 | –/0.06 | ND | ND | |
Aq/Mox | –/6.25 | –/– | –/– | ND | ND | |
S. aureus | Aq/Ctx | –/50 | 100/50 | –/1 | ND | ND |
Aq/R | –/6.25 | 100/50 | –/8 | ND | ND | |
Aq/Of | –/100 | 6.25/3.125 | –/0.25 | ND | ND | |
Aq/Cip | –/12.5 | 50/25 | –/2 | ND | ND | |
Aq/Mox | –/3.125 | 50/25 | –/8 | ND | ND | |
P. aeruginosa | Aq/Ctx | 200/50 | 100/50 | 0.5/1 | 1.5 | Indifference |
Aq/R | 200/6.25 | 12.5/6.25 | 0.06/1 | 1.06 | Indifference | |
Aq/Of | 200/12.5 | 12.5/6.25 | 0.06/0.5 | 0.5 | Synergistic | |
Aq/Cip | 200/3.125 | 25/12.5 | 0.12/4 | 4.1 | Antagonistic | |
Aq/Mox | 200/0.39 | 6.25/3.125 | 0.03/8 | 8.0 | Antagonistic | |
S. aureus (ATCC) | Aq/Ctx | 200/100 | 100/50 | 0.5/0.5 | 1.0 | Indifference |
Aq/R | 200/6.25 | 100/50 | 0.5/8 | 8.5 | Antagonistic | |
Aq/Of | 200/3.12 | 3.125/1.56 | 0.5/0.4 | 0.9 | Additive | |
Aq/Cip | 200/1.56 | 12.5/6.25 | 0.06/4 | 4.06 | Antagonistic | |
Aq/Mox | 200/0.78 | 12.5/6.25 | 0.06/8 | 8.0 | Antagonistic | |
E. coli (ATCC) | Aq/Ctx | –/0.78 | 1.56/0.78 | –/1 | ND | ND |
Aq/R | –/6.25 | 1.56/0.78 | –/0.1 | ND | ND | |
Aq/Of | –/3.12 | 3.125/1.56 | –/0.4 | ND | ND | |
Aq/Cip | –/3.12 | 1.56/0.78 | –/0.2 | ND | ND | |
Aq/Mox | –/0.39 | 12.5/6.25 | –/16 | ND | ND | |