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Table 2 Minimum inhibitory concentration (MIC) and fractional inhibitory concentration (FIC) of n-hexane extract, each antibiotic and their combination against clinical pathogenic and reference bacterial isolates

From: Comprehensive investigation on the synergistic antibacterial activities of Jatropha curcas pressed cake and seed oil in combination with antibiotics

Strain Compound MICa (mg/mL)/MICb (µg/mL) FIC FICI Output
Alone Combined
A. baumannii n-hex/Ctx 100/0.19 1.56/0.78 0.01/4.11 4.1 Antagonistic
n-hex/R 100/6.25 12.5/6.25 0.1/1 1.12 Indifference
n-hex/Of 100/12.5 1.56/0.78 0.01/0.06 0.07 Synergistic
n-hex/Cip 100/6.25 1.56/0.78 0.01/0.1 0.1 Synergistic
n-hex/Mox 100/6.25 12.5/6.25 0.1/1 1.12 Indifference
E. coli n-hex/Ctx 100/100 3.125/1.56 0.03/0.01 0.04 Synergistic
n-hex/R 100/100 0.78/0.39 0.007/0.003 0.01 Synergistic
n-hex/Of 100/50 100/50 1/1 2.0 Indifference
n-hex/Cip 100/100 1.56/0.78 0.01/0.007 0.02 Synergistic
n-hex/Mox 100/3.125 25/12.5 0.25/4 4.25 Antagonistic
E. faecalis n-hex/Ctx –/50 100/50 –/1 ND ND
n-hex/R –/100 100/50 –/0.5 ND ND
n-hex/Of –/12.5 100/50 –/4 ND ND
n-hex/Cip –/50 6.25/3.125 –/0.002 ND ND
n-hex/Mox –/1.56 25/12.5 –/8.01 ND ND
P. vulgaris n-hex/Ctx 100/0.19 1.56/0.78 0.01/0.1 0.1 Synergistic
n-hex/R 100/6.25 0.78/0.39 0.007/0.06 0.07 Synergistic
n-hex/Of 100/12.5 0.78/0.39 0.007/0.03 0.03 Synergistic
n-hex/Cip 100/6.25 0.78/0.39 0.007/0.06 0.07 Synergistic
n-hex/Mox 100/6.25 100/50 1/8 9.0 Antagonistic
S. aureus n-hex/Ctx –/50 –/– –/– ND ND
n-hex/R –/6.25 100/50 –/8 ND ND
n-hex/Of –/50 100/50 –/1 ND ND
n-hex/Cip –/12.5 –/– –/– ND ND
n-hex/Mox –/3.125 12.5/6.25 –/2 ND ND
P. aeruginosa n-hex/Ctx 200/50 12.5/6.25 0.06/0.1 0.1 Synergistic
n-hex/R 200/6.25 3.125/1.56 0.01/0.2 0.2 Synergistic
n-hex/Of 200/12.5 6.25/3.125 0.03/0.25 0.2 Synergistic
n-hex/Cip 200/3.125 3.125/1.56 0.03/0.4 0.5 Synergistic
n-hex/Mox 200/0.39 6.25/3.125 0.03/8.01 8.04 Antagonistic
S. aureus (ATCC) n-hex/Ctx 100/100 25/12.5 0.25/8 8.25 Antagonistic
n-hex/R 100/6.25 6.25/3.125 0.06/0.5 0.5 Synergistic
n-hex/Of 100/3.125 100/50 1/16 17 Antagonistic
n-hex/Cip 100/1.56 0.097/0.048 0.0009/0.03 0.03 Synergistic
n-hex/Mox 100/0.78 6.25/3.125 0.06/4 4.06 Antagonistic
E. coli (ATCC) n-hex/Ctx 100/0.78 3.125/1.56 0.03/0.5 0.5 Synergistic
n-hex/R 100/6.25 0.39/0.195 0.003/0.3 0.03 Synergistic
n-hex/Of 100/3.12 0.39/0.195 0.003/0.06 0.06 Synergistic
n-hex/Cip 100/3.12 0.78/0.39 0.007/0.1 0.1 Synergistic
n-hex/Mox 100/0.39 6.25/3.125 0.06/8 8.0 Antagonistic
  1. MICa minimal inhibitory concentration for n-hexane extract when applied alone, MICb minimal inhibitory concentration for antibiotic when applied alone, Me methanolic extract, Ctx cefotaxime, R rifampicin, Of ofloxacin, Cip ciprofloxacin, Mox moxifloxacin, ND not determined