Fig. 1

Pilot-scale cultivation of IM130005 in the summer. a 100 L flat bioreactor and typical temperature variation inside. The first drop of the temperature is caused by the inlet of CO₂ enriched air and the second drop of the temperature is the result of the end of direct irradiation by the sun. b Using 100 L bioreactor for seeding and 1000 m² race pond cultivation was achieved by two-stage amplification taken place by Shenyang Research Institute of Chemical Industry Co., Ltd