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Table 4 Samples with opposite results between the MNCP assay and rRT-PCR test

From: Evaluation of a novel micro/nanofluidic chip platform for the detection of influenza A and B virus in patients with influenza-like illness

Opposite results

No.

MNCP results (time of positive/minutes)

rRT-PCR results (cycle of threshold)

Positive control

Negative control

Internal control

Influenza A virus

A(H1N1)pdm09

A(H3N2)

Influenza B virus

Influenza A virus

Influenza B virus

Influenza A virus detection

1

+/8.5

+/16.1

+/34.6

2

+/11.2

+/15.2

+/21.4

+/16.8

−/36.1a

3

+/11.2

+/14.7

+/19.2

+/13.1

−/35.2a

4

+/12.0

+/22.1

+/35.6

+/28.6

−/37.6a

5

+/13.4

+/16.4

+/23.9

−/UNDETa

6

+/9.5

+/12.6

+/19.6

−/UNDETa

Influenza B virus detection

1

+/10.5

+/13.6

+/16.1

−/36.3a

2

+/7.9

+/14.4

+/34.8

3

+/8.5

+/20.6

+/30.5

4

+/6.9

+/13.9

+/34.3

5

+/7.7

+/15.7

+/32.5

6

+/14.7

+/18.2

+/32.3

  1. +: positive; −: negative; MNCP: micro/nanofluidic chip platform; rRT-PCR: real-time reverse transcription polymerase chain reaction; UNDET: undetected
  2. aAccording to the reagent instruction, cycle of threshold values of > 34.7/UNDET and > 34.8/UNDET in rRT-PCR tests were interpreted as negative for influenza A virus and influenza B virus, respectively