|
Opposite results
|
No.
|
MNCP results (time of positive/minutes)
|
rRT-PCR results (cycle of threshold)
|
|---|
|
Positive control
|
Negative control
|
Internal control
|
Influenza A virus
|
A(H1N1)pdm09
|
A(H3N2)
|
Influenza B virus
|
Influenza A virus
|
Influenza B virus
|
|---|
|
Influenza A virus detection
|
1
|
+/8.5
|
−
|
+/16.1
|
−
|
−
|
−
|
−
|
+/34.6
|
−
|
|
2
|
+/11.2
|
−
|
+/15.2
|
+/21.4
|
−
|
+/16.8
|
−
|
−/36.1a
|
−
|
|
3
|
+/11.2
|
−
|
+/14.7
|
+/19.2
|
−
|
+/13.1
|
−
|
−/35.2a
|
−
|
|
4
|
+/12.0
|
−
|
+/22.1
|
+/35.6
|
−
|
+/28.6
|
−
|
−/37.6a
|
−
|
|
5
|
+/13.4
|
−
|
+/16.4
|
+/23.9
|
−
|
−
|
−
|
−/UNDETa
|
−
|
|
6
|
+/9.5
|
−
|
+/12.6
|
+/19.6
|
−
|
−
|
−
|
−/UNDETa
|
−
|
|
Influenza B virus detection
|
1
|
+/10.5
|
−
|
+/13.6
|
−
|
−
|
−
|
+/16.1
|
−
|
−/36.3a
|
|
2
|
+/7.9
|
−
|
+/14.4
|
−
|
−
|
−
|
−
|
−
|
+/34.8
|
|
3
|
+/8.5
|
−
|
+/20.6
|
−
|
−
|
−
|
−
|
−
|
+/30.5
|
|
4
|
+/6.9
|
−
|
+/13.9
|
−
|
−
|
−
|
−
|
−
|
+/34.3
|
|
5
|
+/7.7
|
−
|
+/15.7
|
−
|
−
|
−
|
−
|
−
|
+/32.5
|
|
6
|
+/14.7
|
−
|
+/18.2
|
−
|
−
|
−
|
−
|
−
|
+/32.3
|
- +: positive; −: negative; MNCP: micro/nanofluidic chip platform; rRT-PCR: real-time reverse transcription polymerase chain reaction; UNDET: undetected
- aAccording to the reagent instruction, cycle of threshold values of > 34.7/UNDET and > 34.8/UNDET in rRT-PCR tests were interpreted as negative for influenza A virus and influenza B virus, respectively
