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Table 4 Samples with opposite results between the MNCP assay and rRT-PCR test

From: Evaluation of a novel micro/nanofluidic chip platform for the detection of influenza A and B virus in patients with influenza-like illness

Opposite results No. MNCP results (time of positive/minutes) rRT-PCR results (cycle of threshold)
Positive control Negative control Internal control Influenza A virus A(H1N1)pdm09 A(H3N2) Influenza B virus Influenza A virus Influenza B virus
Influenza A virus detection 1 +/8.5 +/16.1 +/34.6
2 +/11.2 +/15.2 +/21.4 +/16.8 −/36.1a
3 +/11.2 +/14.7 +/19.2 +/13.1 −/35.2a
4 +/12.0 +/22.1 +/35.6 +/28.6 −/37.6a
5 +/13.4 +/16.4 +/23.9 −/UNDETa
6 +/9.5 +/12.6 +/19.6 −/UNDETa
Influenza B virus detection 1 +/10.5 +/13.6 +/16.1 −/36.3a
2 +/7.9 +/14.4 +/34.8
3 +/8.5 +/20.6 +/30.5
4 +/6.9 +/13.9 +/34.3
5 +/7.7 +/15.7 +/32.5
6 +/14.7 +/18.2 +/32.3
  1. +: positive; −: negative; MNCP: micro/nanofluidic chip platform; rRT-PCR: real-time reverse transcription polymerase chain reaction; UNDET: undetected
  2. aAccording to the reagent instruction, cycle of threshold values of > 34.7/UNDET and > 34.8/UNDET in rRT-PCR tests were interpreted as negative for influenza A virus and influenza B virus, respectively