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Table 3 Results of the MNCP assay for influenza A and B virus detection and compared with the rRT-PCR test

From: Evaluation of a novel micro/nanofluidic chip platform for the detection of influenza A and B virus in patients with influenza-like illness

Virus

No. detected (MNCP/rRT-PCR)a

Sensitivity (%) (95% CI)

Specificity (%) (95% CI)

PPVs (%) (95% CI)

NPVs (%) (95% CI)

Overall concordance rate (%) (95% CI)

P value (McNemar’s test)

Kappa value (95% CI)

+/+

+/−

−/+

−/−

Influenza A

89

5

1

160

98.89 (93.10 − 99.94)

96.97 (92.70 − 98.88)

94.68 (87.45 − 98.03)

99.38 (96.07 − 99.97)

97.65 (94.70 − 99.04)

0.219

0.95** (0.91 − 0.99)

Influenza B

94

1

5

159

94.95 (88.06 − 98.13)

99.38 (96.04 − 99.97)

98.95 (93.44 − 99.95)

96.95 (92.65 − 98.87)

97.68 (94.78 − 99.05)

0.219

0.95** (0.91 − 0.99)

  1. +: positive; −: negative; MNCP: micro/nanofluidic chip platform; rRT-PCR: real-time reverse transcription polymerase chain reaction; CI: confidence interval; PPV: positive predictive value; NPV: negative predictive value
  2. **P < 0.001 (kappa statistics)
  3. aMNCP and rRT-PCR were conducted successively in 255 of 266 patients to detect influenza A virus (11 were excluded because of sample volume shortage) and in 259 of 266 patients to detect influenza B virus (seven excluded because of sample volume shortage)