Evaluation of a novel micro/nanofluidic chip platform for the detection of influenza A and B virus in patients with influenza-like illness

AMB Express

Table 1 The primer and beacon probe sequences

Subtypes of influenza viruses	Primer or probe	Sequence (5′-3′)	Length (bp)
Influenza A MP	Forward primer	CACTDGGCACGGTGAGCGTGAA	201
	Reverse primer	AATTCTAATACGACTCACTATAGGGAGATTCTAACCGAGGTCGAAACG
	Beacon probe	FAM-CCCGTCTTTAGCCAYTCCATGAGAGCCTCACGGG-BHQ1
Influenza A(H1N1)pdm09 HA	Forward primer	ATGATAATACCAGATCCAGCA	173
	Reverse primer	AATTCTAATACGACTCACTATAGGGAGAGTTCAAGCCGGAAATAGCAAT
	Beacon probe	FAM-CCCGTAATGCATATCTCGGTACCACTAGATTTCACGGG-BHQ1
Influenza A(H3N2) HA	Forward primer	CATAAGGGTAACAGTTGCT	188
	Reverse primer	AATTCTAATACGACTCACTATAGGGAGAATGCTACTGAGCTGGTTCA
	Beacon probe	FAM-CGCGTTTGAGGGTCTCCCAATAGAGCATCACGCG
Influenza B MP	Forward primer	CCAAAACTGTTTCACCCATT	168
	Reverse primer	AATTCTAATACGACTCACTATAGGGAGAACAATAAACAGAGAGGTATC
	Beacon probe	FAM-CCCGTTCAATACCTCCATGTTGTCAGAGAGTACGGG-BHQ1

Four pairs of primers and molecular beacon probes specific for influenza A virus matrix protein (MP) gene, influenza A(H1N1)pdm09 hemagglutinin (HA) gene, influenza A(H3N2) HA gene, and influenza B virus MP gene were employed to detect four subtypes of influenza viruses