Fig. 6

Synazol red 6HBN dye enters into the cell (A. aquatilis 3c) by unknown mechanism. Upon entrance into the cell the dye is enzymatically processed into various end products. Most probably, azo group (–N=N–) is reduced, followed by cleavage reaction and different end products are produced. Secondly, desulfonation and oxidative deamination results in synthesis of pyrrolo[1,2-a]pyrazine-1,4-dione derivative which can be used as substrates in amino acid metabolism. The amino acid catabolism can synthesize pyruvate (3C compound) which can be converted into acetyl-CoA. The acetyl-CoA undergoes Krebs cycle to produce NADH₂ and FADH₂ (substrates of electron transport chain). Moreover, dye desulfonation, oxidative deamination and carboxylation lead to produce phthalate derivatives, which can be transformed into different fatty acids and aldehydes. The phthalate, fatty acids and aldehydes can directly/indirectly enter into fatty acid oxidation reactions (β-oxidation) to produce acetyl-CoA, NADH₂ and FADH₂