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AMB Express
Fig. 2 | AMB Express

Fig. 2

From: Expression of benzoyl-CoA metabolism genes in the lignocellulolytic host Caldicellulosiruptor bescii

Fig. 2

Western blot image of whole-cell lysate from C. bescii expression strain JWCB018 pJOT1 showing a protein product of the size expected for heterologously expressed F. placidus benzoyl-CoA ligase protein as the major detected product. Whole-cell lysate was prepared, electrophoresed, and probed using anti-6× histidine antibody (see methods). Lane 1: MagicMark XP Western Protein Standard (ThermoFischer Scientific, Waltham, MA) protein masses (in kDa) indicated adjacent to each band, Lane 2: Lysate from strain JWCB018 pJOT1, Lane 3: Lysate from strain JWCB018 pJGW07 (hosting an empty expression vector)

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