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Table 1 Short summary of bacteriophage-based rapid bacteria detection studies using genetically engineered bacteriophage T7

From: Rapid detection of Escherichia coli in beverages using genetically engineered bacteriophage T7

Method description Detection limit Assay time Matrices Potential limitations References
Detection of T7-induced alkaline phosphatase activity using chemilumiscent methods 103 CFU/ml 6 h Luria Broth Detection of enzymatic activity can be affected by background noise Alcaine et al. (2015)
Detection of phage T7 amplification using lateral flow assays with phage-based enzymatic reporter 102CFU/100 ml 9 h River water Reduced detection efficacy in complex matrices may occur due to non-specific binding to antibody Alcaine et al. (2016)
Detection of T7-induced β-galactosidase using colorimetric substrate 102 CFU/ml 7 h Drinking water, skim milk, orange juice Additional cost and complexity due to lyophilization Chen et al. (2017)
Detection of T7-induced β-galactosidase using electrochemical method 102 CFU/ml 7 h Drinking water, skim milk, orange juice Low sample volume (1 ml) Wang et al. (2017)
Detection of T7-induced luciferase and alkaline phosphatase by filter-based colorimetric and bioluminescence method 1 CFU/100 ml 10 h Drinking water Reduced detection efficacy in complex matrices may occur due to background color and large food particles Hinkley et al. (2018b)
Detection of T7-induced luciferase immobilized on microcrystalline cellulose < 10 CFU per 100 ml 3 h Drinking water Reduced detection efficacy in complex matrices may occur due to non-specific binding to cellulose Hinkley et al. (2018a)