Rapid detection of Escherichia coli in beverages using genetically engineered bacteriophage T7

AMB Express

Table 1 Short summary of bacteriophage-based rapid bacteria detection studies using genetically engineered bacteriophage T7

Method description	Detection limit	Assay time	Matrices	Potential limitations	References
Detection of T7-induced alkaline phosphatase activity using chemilumiscent methods	10³ CFU/ml	6 h	Luria Broth	Detection of enzymatic activity can be affected by background noise	Alcaine et al. (2015)
Detection of phage T7 amplification using lateral flow assays with phage-based enzymatic reporter	10²CFU/100 ml	9 h	River water	Reduced detection efficacy in complex matrices may occur due to non-specific binding to antibody	Alcaine et al. (2016)
Detection of T7-induced β-galactosidase using colorimetric substrate	10² CFU/ml	7 h	Drinking water, skim milk, orange juice	Additional cost and complexity due to lyophilization	Chen et al. (2017)
Detection of T7-induced β-galactosidase using electrochemical method	10² CFU/ml	7 h	Drinking water, skim milk, orange juice	Low sample volume (1 ml)	Wang et al. (2017)
Detection of T7-induced luciferase and alkaline phosphatase by filter-based colorimetric and bioluminescence method	1 CFU/100 ml	10 h	Drinking water	Reduced detection efficacy in complex matrices may occur due to background color and large food particles	Hinkley et al. (2018b)
Detection of T7-induced luciferase immobilized on microcrystalline cellulose	< 10 CFU per 100 ml	3 h	Drinking water	Reduced detection efficacy in complex matrices may occur due to non-specific binding to cellulose	Hinkley et al. (2018a)