Table 2 List of primer set used in this study
Target species | Molecular assay | Primer code | Sequence | References |
|---|---|---|---|---|
Phytophthora ramorum | LAMP | Phy-r_F3 | 5′-ACGTTGTTGGTTGTGGAG-3′ | This study |
Phy-r_B3 | 5′-CCAATTGAGATGCCAGCA-3′ | |||
Phy-r_FLP | 5′-CGCATTGTTCAGCCGAAG-3′ | |||
Phy-r_BLP | 5′-GAATCGACGGTGTTGTGC-3′ | |||
Phy-r_FIP | 5′-AGTCATTACCGCCACAGCAGTGTTCGATTCGCGGTA-3′ | |||
Phy-r_BIP | 5′-CGTAGCTGTGCAGGGCTTGAACCGCCACTCTACTTC-3′ | |||
qPCR | PramF | 5′-GCAGGGCTTGGCTTTTGA-3′ | Migliorini et al. (2018) | |
PramR | 5′-GCCGAACCGCCACTCTACT-3′ | |||
Pram_PR | 5′-FAM-TCGACGGTGTTGTGCG-MGBNFQ-3′ | |||
Xylella fastidiosa | LAMP | XF_F3 | 5′-TAGAGTCTTGGACTGAGCC-3′ | This study |
XF_B3 | 5′-ATCGACCCAGTAATACTCGT-3′ | |||
XF_FLP | 5′-AGGAGAACGTAATAACCACGG-3′ | |||
XF_BLP | 5′-TCCTGGCATCAATGATCGTAAT-3′ | |||
XF_FIP | 5′-CACCATTCAACATGGACTCGGTGCGATCTTCCGTTACCAG-3′ | |||
XF_BIP | 5′-CTACGAGACTGGCAAGCGTTCGTACCACAGATCGCTTC-3′ | |||
qPCR | Xf_Fw | 5′-CGGGTACCGAGTCCATGTTG-3′ | This study | |
Xf_Rev | 5′-CAATCAAACGCTTGCCAGTCT-3′ | |||
Xf_Pr | 5′-FAM-TGGTGCCCGTGGCTA-MGBNFQ-3′ | |||
Ceratocystis platani | LAMP | CPL_F3 | 5′-CAGCGAAATGCGATAAGTAATG-3′ | This study |
CPL_B3 | 5′-TTTATACTACACAGGGGAGTTG-3′ | |||
CPL_FIP | 5′-AATGACGCTCGGACAGGCTCGAATCTTTGAACGCACA-3′ | |||
CPL_BIP | 5′-TGTTCTTGGCGTTGGAGGTCGCAAGTATAACAGCCGATACA-3′ | |||
CPL_FLP | 5′-TGCCTGGCAGAATACTGC-3′ | |||
CPL_BLP | 5′-GTTCTCCCCTGAACAGGC-3′ | |||
qPCR | CpITS-F | 5′-GCCTGTCCGAGCGTCATT-3′ | Luchi et al. (2013) | |
CpITS-R | 5′-CCTCCAACGCCAAGAACAAA-3′ | |||
CpITS-Pr | 5′-FAM-CACCACTCAAGGACTC-MGB-3′ | |||
Cytochrome oxidase (COX) endogenous plant gene | LAMP | COX F3 | 5′-TATGGGAGCCGTTTTTGC-3′ | |
COX B3 | 5′-AACTGCTAAGRGCATTCC-3′ | |||
COX FLP | 5′-ATGTCCGACCAAAGATTTTACC-3′ | |||
COX BLP | 5′-GTATGCCACGTCGCATTCC-3′ | |||
COX FIP | 5′-ATGGATTTGRCCTAAAGTTTCAGGGCAGGATTTCACTATTGGGT-3′ | |||
COX BIP | 5′-TGCATTTCTTAGGGCTTTCGGATCCRGCGTAAGCATCTG-3′ |