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Table 2 List of primer set used in this study

From: Real-time loop-mediated isothermal amplification: an early-warning tool for quarantine plant pathogen detection

Target species Molecular assay Primer code Sequence References
Phytophthora ramorum LAMP Phy-r_F3 5′-ACGTTGTTGGTTGTGGAG-3′ This study
Phy-r_B3 5′-CCAATTGAGATGCCAGCA-3′
Phy-r_FLP 5′-CGCATTGTTCAGCCGAAG-3′
Phy-r_BLP 5′-GAATCGACGGTGTTGTGC-3′
Phy-r_FIP 5′-AGTCATTACCGCCACAGCAGTGTTCGATTCGCGGTA-3′
Phy-r_BIP 5′-CGTAGCTGTGCAGGGCTTGAACCGCCACTCTACTTC-3′
qPCR PramF 5′-GCAGGGCTTGGCTTTTGA-3′ Migliorini et al. (2018)
PramR 5′-GCCGAACCGCCACTCTACT-3′
Pram_PR 5′-FAM-TCGACGGTGTTGTGCG-MGBNFQ-3′
Xylella fastidiosa LAMP XF_F3 5′-TAGAGTCTTGGACTGAGCC-3′ This study
XF_B3 5′-ATCGACCCAGTAATACTCGT-3′
XF_FLP 5′-AGGAGAACGTAATAACCACGG-3′
XF_BLP 5′-TCCTGGCATCAATGATCGTAAT-3′
XF_FIP 5′-CACCATTCAACATGGACTCGGTGCGATCTTCCGTTACCAG-3′
XF_BIP 5′-CTACGAGACTGGCAAGCGTTCGTACCACAGATCGCTTC-3′
qPCR Xf_Fw 5′-CGGGTACCGAGTCCATGTTG-3′ This study
Xf_Rev 5′-CAATCAAACGCTTGCCAGTCT-3′
Xf_Pr 5′-FAM-TGGTGCCCGTGGCTA-MGBNFQ-3′
Ceratocystis platani LAMP CPL_F3 5′-CAGCGAAATGCGATAAGTAATG-3′ This study
CPL_B3 5′-TTTATACTACACAGGGGAGTTG-3′
CPL_FIP 5′-AATGACGCTCGGACAGGCTCGAATCTTTGAACGCACA-3′
CPL_BIP 5′-TGTTCTTGGCGTTGGAGGTCGCAAGTATAACAGCCGATACA-3′
CPL_FLP 5′-TGCCTGGCAGAATACTGC-3′
CPL_BLP 5′-GTTCTCCCCTGAACAGGC-3′
qPCR CpITS-F 5′-GCCTGTCCGAGCGTCATT-3′ Luchi et al. (2013)
CpITS-R 5′-CCTCCAACGCCAAGAACAAA-3′
CpITS-Pr 5′-FAM-CACCACTCAAGGACTC-MGB-3′
Cytochrome oxidase (COX) endogenous plant gene LAMP COX F3 5′-TATGGGAGCCGTTTTTGC-3′ Tomlinson et al. (2010a, b)
COX B3 5′-AACTGCTAAGRGCATTCC-3′
COX FLP 5′-ATGTCCGACCAAAGATTTTACC-3′
COX BLP 5′-GTATGCCACGTCGCATTCC-3′
COX FIP 5′-ATGGATTTGRCCTAAAGTTTCAGGGCAGGATTTCACTATTGGGT-3′
COX BIP 5′-TGCATTTCTTAGGGCTTTCGGATCCRGCGTAAGCATCTG-3′