From: A novel method for the capture-based purification of whole viral native RNA genomes
Strategy type | Employed in | Advantages | Disadvantages |
---|---|---|---|
1. Custom adapter | – Keller et al. (2018) | – Highest sequencing specificity – Rapid turnaround time | – Cannot sequence fragmented RNA – Foreknowledge of target sequence required |
2. Conventional adapter | – Wongsurawat et al. (2019) – Pre-capture group | – Sequences fragmented RNA – No foreknowledge of target sequence – Rapid turnaround time | – Low sequencing specificity |
3. Conventional adapter + capture | – Post-capture group Concentrated post capture group | – Sequences fragmented RNA – Less foreknowledge of target sequence required – Higher sequencing specificity | – Increased turnaround time |
4. Conventional adapter + host rRNA depletion | – Wongsurawat et al. (2019) | – Sequences fragmented RNA – No foreknowledge of target sequence – Fast turnaround time | – Low sequencing specificity |