Table 1 The table below gives an overview of the enzymes used in this study
Enzyme | NCBI or Uniprot identifier | Function | EC number | Microbial origin | Purification tag included | Expression host (used in this work) |
|---|---|---|---|---|---|---|
Cc XylB | GI: 614102613 | Dehydrogenase | 1.1.1.175 | Caulobacter crescentus | Strep-II + TEV_N | S. cerevisiae |
Cc XylC | CC_0820 GI: 16125073 | Lactonase | 3.1.1.68 | C. crescentus | No tag | E. coli |
Cc XylD | GI: 1043567249 | Dehydratase | 4.2.1.82 | C. crescentus | Strep-II_N | E. coli |
Ec YagE | b0268 GI: 357529065 | Aldolase | 4.1.2.20 | E. coli | Strep-II_N | E. coli |
Ec AldA | b1415 GI: 113602 | Oxidoreductase | 1.2.1.22 | E. coli | His6-tag_N | E. coli |
Ab AraE | GI: 40339944 | Oxidoreductase | 1.2.1.24 1.2.1.26 | Azospirillum brasieliense | His6-tag_N | E. coli |
Ab α-KGSA DH | GI: 81613403 | Dehydrogenase | 1.2.1.26 | Acinetobacter baylyii ADP1 | His6-tag_N | E. coli |
Ec YqhD | b3011 GI: 301015215 | Oxidoreductase | 1.1.1.- | E. coli | His6-tag_N | E. coli |
LDH | Commercial | Dehydrogenase | 1.1.1.27 | Rabbit muscle | – | – |
Ec FucO | b2799 GI: 357528800 | Oxidoreductase | 1.1.1.77 | E. coli | His6-tag_C | E. coli |
Hm XylD | dgoA4, GI: 55230170 | Dehydratase | 4.2.1.82 | Haloarcula marismortui | Strep-II_N | E. coli |
Hv XylD | GI: 292493977 | Dehydratase | 4.2.1.82 | Haloferax volcanii | Strep-II_N | E. coli |
Se GluDHT | GI: 667467043 | Dehydratase | 4.2.1.- | Salmonella enterocolica | Strep-II_N | E. coli |
Rx MR/MLE | GI: 123368307 | Dehydratase | 4.2.1.- | Rubrobacter xylanophilus | His6-tag_C | E. coli |
Pa GalDHT | UniProt: D4GJ14 | Dehydratase | 4.2.1.- | Pantoea ananatis | His6-tag_N | E. coli |