Fig. 8

Evaluation of the final dehydrogenase of the in vitro enzymatic pathway from d-xylose to glycolic acid. The in vitro pathway is composed of 5 enzymatic steps (Cc XylB, Cc XylC, Cc XylD, Ec YagE and Ec AldA or Ab AraE). The production of glycolate was followed through an enzymatic assay using HAO1, which converts glycolate to glyoxylate and produced H₂O₂, which can be detected using HRP and Amplex red. The assay was performed as a two-step process as described in “Materials and methods”. The two final dehydrogenases of the pathway Ec AldA and Ab AraE are compared using this method