Table 1 List of RT-PCR primers used for the amplification of TNF-α, IL-6, MMP-2 and MMP-9
S. no | Gene name | Forward | Reverse |
|---|---|---|---|
1 | TNF-α | 5′-CCCAGACCCTCACACTCAGAT-3′ | 5′-TTG TCC CTTGAA GAG AAC CTG-3′ |
2 | IL-6 | 5′-AAGTTTCTCTCCGCAAGATAC TTCCAGCCA-3′ | 5′-AGG CAAATTTCCTGGTTATATCCA GTTT-3′ |
3 | MMP-2 | 5′-AGGATCATTGGCTACACACC-3′ | 5′-AGCTGTCATAGGATGTGCCC-3′ |
4 | MMP-9 | 5′-CGCAGACATCGTCATCCAGT-3′ | 5′-GGATTGGCCTTGGAAGATGA-3′ |
5 | GAPDH | 5′-TCCCTCAAGATTGTCAGCAA-3′ | 5′-AGATCCACAACGGATACATT-3′ |