Table 2 The primers used in this study
Primer | Sequence and modifications (5′–3′) | Length |
|---|---|---|
F1 | GCTGCTTTTTCCACTCGTT | 19 nt |
CP1 | TCACTTCTGAAACATGATCGACCTATGGATTTTTGAAGGGCAC | 43 mer |
C1 | TCACTTCTGAAACATGATCGACC | 23 nt |
D1 | CACACATAGTCATAAATGAG | 20 nt |
D1*a | FITC-CACACATAGTCATAAATGAG | 20 nt |
R1 | CCTATTAACAGAAGTAAATGC | 21 nt |
R1*b | Biotin-CCTATTAACAGAAGTAAATGC | 21 nt |
R2 | TCTGAGCGCGCTCATTT | 17 nt |
D2 | GATGATCGTCGCAGCAA | 17 nt |
C2 | ATGGGCAAGTTATAACTGGCTTAG | 24 nt |
CP2 | ATGGGCAAGTTATAACTGGCTTAGAACAGAGCTATTCGGGGC | 42 mer |
F2 | ATTGTGAGGCCAGCAACT | 18 nt |