Fig. 4

The detection limit of A. baumanni-MCDA-LFB assays. Three measurement techniques, including colorimetric indicator (a), gel electrophoresis (b) and lateral flow biosensor (c), were applied for analysis of MCDA amplicons. Serial dilutions of the template (10 ng, 100 pg, 10 pg, 1 pg, 100 fg, 10 fg and 1 fg) were subjected to standard MCDA reactions. Tubes (a), lanes (b), biosensors (c) 1–8 respectively represent A. baumannii strain ATCC19606 DNA levels of 10 ng, 100 pg, 10 pg, 1 pg, 100 fg, 10 fg and 1 fg per reaction, and a blank control (DW)