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Table 4 Ascomycin production by different S. hygroscopicus strains

From: Enhancement of ascomycin production via a combination of atmospheric and room temperature plasma mutagenesis in Streptomyces hygroscopicus and medium optimization

Strains (S. hygroscopicus) Methods Scale Ascomycin (mg/L) References
FS35 Titanium sapphire laser systema 1 L flask 305.6 Qi et al. (2012)
SA68 Titanium sapphire laser system and shikimic acid enduring screening modelb 1 L flask 450.0 Qi et al. (2014)
HA-Hcd-Ccr Overexpression of hcd and ccrc 1 L flask 438.9 Wang et al. (2017)
OfkbRE Overexpression of fkbR1 and fkbEd 1 L flask 536.7 Song et al. (2017)
TD-ΔPyc-FkbO Overexpressing fkbO and inactivating Pyce 3 L fermenter 610.0 Qi et al. (2017)
SFK-36 ARTP mutagenesis and medium optimization 5 L fermenter 1476.9 This study
  1. aThe FS35 mutant strain was obtained in the optimal irradiation conditions (25 mW for 6 min) by the Titanium sapphire laser system (810 nm, 76 MHz, 150 fs)
  2. bTitanium sapphire laser system (25 mW for 6 min) was combined with an enduring selection mode containing 5 g/L shikimic acid to generate SA68 mutant strain
  3. cFunctional gene hcd and ccr were overexpressed together under the control of promoter PermE* and integrated into ΦC31 integration site of FS35 strain to generate engineering strain HA-Hcd-Ccr
  4. dPositive regulatory gene fkbR1 and functional gene fkbE were overexpressed together under the control of PermE* and integrated into ΦC31 integration site of FS35 strain
  5. eGene deletion plasmid pΔPyc derived from pKC1139 was used for inactivating Pyc gene in SA68 strain to generate engineering strain TD-ΔPyc. And then, fkbO gene under the control of PermE* was integrated into ΦC31 integration site of strain TD-ΔPyc through conjugal transfer