Enhancement of ascomycin production via a combination of atmospheric and room temperature plasma mutagenesis in Streptomyces hygroscopicus and medium optimization

AMB Express

Table 3 ANOVA for response surface quadratic model

Source	Sum of square	Degrees of freedom	Mean square	F-value	P-value Prob > F	Significant
Model	99,2600.00	9	110,300.00	74.75	< 0.0001	***
A	3228.06	1	3228.06	2.19	0.1826
B	165400.00	1	165400.00	112.13	< 0.0001	***
C	56229.81	1	56229.81	38.11	0.0005	***
AB	19572.01	1	19572.01	13.28	0.0083	**
AC	14677.32	1	14677.32	9.95	0.0161	*
BC	31.36	1	31.36	0.021	0.8882
A²	84259.53	1	84259.53	57.11	0.0001	***
B²	349000.00	1	349000.00	236.54	< 0.0001	***
C²	229900.00	1	229900.00	155.82	< 0.0001	***
Residual	10327.10	7	1475.30
Lack of Fit	8026.54	3	2675.51	4.65	0.0858
Pure error	2300.56	4	575.14
Cor Total	1003000.00	16

* P < 0.05; **P < 0.01; ***P < 0.001; C.V. % = 3.46; R² = 0.99; Adj R² = 0.98; Pred R² = 0.87