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Table 2 Experimental design for media optimization

From: Enhancement of ascomycin production via a combination of atmospheric and room temperature plasma mutagenesis in Streptomyces hygroscopicus and medium optimization

Run

A

B

C

Ascomycin (mg/L)

1

− 1

0

− 1

852.1

2

− 1

1

0

899.4

3

0

− 1

− 1

954

4

− 1

0

1

1162.6

5

0

0

0

1435.8

6

1

0

− 1

1054.9

7

0

0

0

1397.5

8

1

0

1

1123.1

9

0

1

− 1

703.5

10

1

1

0

758.2

11

1

− 1

0

1228.4

12

0

− 1

1

1094.4

13

0

0

0

1416.2

14

0

1

1

855.1

15

0

0

0

1458.2

16

0

0

0

1408.8

17

− 1

− 1

0

1089.8