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Table 1 Comparison of the steady-state kinetic parameters of LAC_2.9 with those of laccases from Thermus thermophilus strains

From: A thermostable laccase from Thermus sp. 2.9 and its potential for delignification of Eucalyptus biomass

 

ABTS

DMP

Km (mM)

kcat (s−1)

kcat/Km (mM−1 s−1)

Km (mM)

kcat (s−1)

kcat/Km (mM−1 s−1)

LAC_2.9a

0.59 ± 0.08

1.00 ± 0.04

1.7 ± 0.2

0.119 ± 0.006

11.6 ± 0.3

97 ± 3

Tth-laccaseb

0.9

24.6

27.3

naf

na

na

Tth-laccasec

2.4

4.77

1.98

na

na

na

TtSLACd

0.49

1.48

3.02

0.11

2.9

27

LacTTe

0.036 ± 0.003

0.37 ± 0.01

10.13 ± 0.03

0.15 ± 0.01

0.13 ± 0.01

1.00 ± 0.03

  1. aReactions at 60 °C. For ABTS: 20 mM sodium acetate, pH 5. For DMP: 20 mM sodium phosphate, pH 6
  2. bMiyazaki (2005). Reactions at 90 °C. Buffer information not available
  3. cKumari et al. (2018). Reactions at 60 °C. Buffer Britton and Robinson, pH 4.5
  4. dKim et al. (2015). Reactions at 75 °C. For ABTS: 50 mM sodium acetate, pH 4.5. For DMP: buffer information not available
  5. eLiu et al. (2015). Reactions at 90 °C. For ABTS: buffer Britton and Robinson, pH 4.5. For DMP: buffer Britton and Robinson, pH 8
  6. fNot available