Fig. 2

Dependence of LAC_2.9 activity on reaction conditions. a Copper dependence of activity; b pH dependence of activity; c thermal stability profile obtained measuring the residual activity on ABTS at 60 °C, after incubating the enzyme at 60, 70 and 80 °C up to 16 h; d effect of metal ions, SDS, EDTA, DTT (1 mM of each) and NaCl (1–100 mM). Assays were carried out in triplicate. The bars indicate standard deviation