Fig. 1From: A thermostable laccase from Thermus sp. 2.9 and its potential for delignification of Eucalyptus biomassExpression and purification of LAC_2.9. Denaturing gel was loaded with: 1—recombinant E. coli culture induced with 1 mM IPTG; 2—recombinant E. coli crude extract of soluble proteins; 3—purified LAC_2.9 using Ni–NTA affinity chromatography; and 4—molecular weight standard (Promega, Madison, WI, USA)Back to article page